Thermophoresis for characterizing biomolecular interaction
نویسندگان
چکیده
منابع مشابه
Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions.
Microscale thermophoresis (MST) allows for quantitative analysis of protein interactions in free solution and with low sample consumption. The technique is based on thermophoresis, the directed motion of molecules in temperature gradients. Thermophoresis is highly sensitive to all types of binding-induced changes of molecular properties, be it in size, charge, hydration shell or conformation. I...
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Abstract The use of infrared laser sources for creation of localized temperature fields has opened new possibilities for basic research and drug discovery. A recently developed technology, Microscale Thermophoresis (MST), uses this temperature field to perform biomolecular interaction studies. Thermophoresis, the motion of molecules in temperature fields, is very sensitive to changes in size, c...
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ژورنال
عنوان ژورنال: Methods
سال: 2018
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2018.02.003